This fully updated, self-contained textbook covering modern optical microscopy equips students with a solid understanding of the theory underlying a range of advanced techniques. Two new chapters cover pump-probe techniques, and imaging in scattering media, and additional material throughout covers light-sheet microscopy, image scanning microscopy, and much more. An array of practical techniques are discussed, from classical phase contrast and confocal microscopy, to holographic, structured illumination, multi-photon, and coherent Raman microscopy, and optical coherence tomography. Fundamental topics are also covered, including Fourier optics, partial coherence, 3D imaging theory, statistical optics, and the physics of scattering and fluorescence. With a wealth of end-of-chapter problems, and a solutions manual for instructors available online, this is an invaluable book for electrical engineering, biomedical engineering, and physics students taking graduate courses on optical microscopy, as well as advanced undergraduates, professionals, and researchers looking for an accessible introduction to the field.Read more
- Self-contained, covering fundamental physical theory as well as practical applications
- Provides comprehensive coverage of both classical and modern optical microscopy techniques
- Includes numerous end-of-chapter problems, with a solutions manual for instructors available online
Reviews & endorsements
'Of the many books available on microscopy, my favorite has been Introduction to Optical Microscopy by Jerome Mertz. Now this excellent book is available in an even better second edition.' Joseph W. Goodman, Stanford University, CaliforniaSee more reviews
'For advanced undergraduates, graduate students, researchers and engineers who want to gain indepth understanding of the principles of optical microscopy or develop their own microscopes, this book is a must-have.' Yang Liu, University of Pittsburgh
'No other book covers microscopy theory as broadly as Mertz’s, nor with his consistent focus on ts theoretical underpinnings. His book fills a clear pedagogical gap in the literature, and is an excellent resource for professionals as well as students new to the field.' Neil Switz, San Jose State University
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- Edition: 2nd Edition
- Publication planned for: September 2019
- format: Hardback
- isbn: 9781108428309
- length: 462 pages
- dimensions: 252 x 195 x 25 mm
- weight: 1.18kg
- contains: 164 b/w illus. 192 exercises
- availability: Not yet published - available from September 2019
Table of Contents
2. Monochromatic wave propagation
3. Monochromatic field propagation through lens
4. Intensity propagation
5. 3D imaging
7. Intensity fluctuations
8. Detection noise
9. Absorption and scattering
10. Widefield microscopy
11. Interference microscopy
12. Optical coherence tomography
14. Confocal microscopy
15. Structured illumination microscopy
16. Multiphoton microscopy
17. Multiharmonic microscopy
18. Pump-probe microscopy
20. Imaging in scattering media
Appendix A. Properties of Fourier transforms
Appendix B. Miscellaneous math
Appendix C. Jones matrix description of polarization.
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