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Introduction to Flow Cytometry

$61.00 ( ) USD

  • Author: James V. Watson, MRC Clinical Oncology Unit, The Medical School, Cambridge
  • Date Published: March 2011
  • availability: This ISBN is for an eBook version which is distributed on our behalf by a third party.
  • format: Adobe eBook Reader
  • isbn: 9780511876509

$ 61.00 USD ( )
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  • Flow cytometry is a technique for measuring both scattered light and fluorescence from single cells at very rapid rates. Typically up to 5000 cells can be analysed per second. Using various fluorochromes this allows a cell population to be analysed for cells showing certain characteristics such as the presence of a particular enzyme, cellular constituent or other gene product. The information it can provide is invaluable in helping to diagnose certain cancers as well as aiding pure research into many aspects of cell biology such as the cell cycle and gene expression. This book describes the technology in a simple and direct way. The fundamental concepts upon which the technology is based are outlined and the book goes on to describe flow cytometers and what can be gained from using them in biology and medicine. The book gives a particularly detailed account of how artifactual results can arise and where 'noise' is generated. Anyone starting to use, or already using this technique, will need to read this book.

    • The technology is described in a simple, direct manner
    • Fundamental concepts are outlined
    • Will be necessary reading for practitioners
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    Product details

    • Date Published: March 2011
    • format: Adobe eBook Reader
    • isbn: 9780511876509
    • availability: This ISBN is for an eBook version which is distributed on our behalf by a third party.
  • Table of Contents

    Acknowledgements
    1. Introduction
    2. Fluid flow dynamics
    3. Light and optics
    4. Electronics
    5. Computing
    6. Cell sorting
    7. Preparation and staining
    8. Miscellaneous techniques
    9. Instrument performance
    10. Light scatter applications
    11. Nucleic acid analysis
    12. Nucleic acids and protein
    13. Chromosomes
    14. Dynamic cellular events
    15. Applications in oncology
    16. Epilogue
    References
    Index.

  • Author

    James V. Watson, MRC Clinical Oncology Unit, The Medical School, Cambridge

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