In Situ Hybridisation
Application to Developmental Biology and Medicine
$48.00 ( ) USD
- N. Harris, University of Durham
- D. G. Wilkinson, National Institute for Medical Research, London
Adobe eBook Reader
Looking for an examination copy?
If you are interested in the title for your course we can consider offering an examination copy. To register your interest please contact firstname.lastname@example.org providing details of the course you are teaching.
Advances in our understanding of biological mechanisms have frequently been associated with the development of new techniques. In situ hybridization is a classic case of just such an advance. The technique effectively combines histochemistry with molecular biology and enables the rapid analysis of the distribution of RNA, or DNA, in the tissues. The information gained from this has caused something of a revolution in our understanding of developmental biology, since a fundamental aspect of development is the spatial and temporal expression of genes. In addition the technique has found application in the field of medicine, where it is giving new insights into the functioning of healthy tissues and the diagnosis and study of diseases. This book brings together contributions from leaders in the application of this technique and will help the reader review and be guided through the various options and variations of the technique.
Reviews & endorsements
"...very useful for biologists, molecular biologists, biophysicists, and advanced students." Journal of Steroid Biochemistry and Molecular Biology
Not yet reviewed
Be the first to review
Review was not posted due to profanity×
- Date Published: May 2012
- format: Adobe eBook Reader
- isbn: 9781139242721
- availability: This ISBN is for an eBook version which is distributed on our behalf by a third party.
Table of Contents
1. Non radio isotopic labels for in situ hybridisation histochemistry: a histochemist's view G. Coulton
2. Use of haptenised nucleic acid probes in fluorescent in situ hybridisation A. K. Raap
3. The use of complementary RNA probes for the identification and localisation of peptide messenger RNA in the diffuse neuroendocrine system A. Giaid
4. Contributions of the spatial analysis of gene expression to the study of sea urchin development R. C. Aangerer
5. Advantages and limitations of in situ hybridisation as exemplified by the molecular genetic analysis of Drosophila development P. W. Ingham
6. The use of in situ hybridisation to study the localisation of maternal mRNAs during Xenopus oogenesis H. Perry-O'Keefe
7. In situ hybridisation in the analysis of genes with potential roles in mouse embryogenesis D. G. Wilkinson
8. Evolution of algal plasmids from eukaryotic endosymbionts G. I. McFadden
9. Localisation of expression of male flower specific genes from maize by in situ hybridisation S. Y. Wright and A. J. Greenland
10. Tissue preparation techniques for in situ hybridisation studies of storage-protein gene expression during pea seed development N. Harris
11. Investigation of gene expression during plant gametogenesis by in situ hybridisation K. G. Jones
12. Sexing the human conceptus by in situ hybridisation J. D. West
13. Nonisotopic in situ hybridisation in human pathology C. S. Herrington
14. The demonstration of viral DNA in human tissues by in situ hybridisation M. Wells.
Sorry, this resource is locked
Please register or sign in to request access. If you are having problems accessing these resources please email email@example.comRegister Sign in
You are now leaving the Cambridge University Press website. Your eBook purchase and download will be completed by our partner www.ebooks.com. Please see the permission section of the www.ebooks.com catalogue page for details of the print & copy limits on our eBooks.Continue ×